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These online “how to” guides include selected guides from leading public institutions and universities.

This course is designed for new and experienced users of PubMed®, the National Library of Medicine (NLM®) journal literature search system.

By the end of this course, you should be able to:

  • Understand PubMed's scope and content.
  • Understand how the MeSH vocabulary is used to describe and retrieve citations.
  • Build a search using MeSH and PubMed search tools.
  • Manage your results using display, sort, the Clipboard, save, print, e-mail and order features and My NCBI filters.
  • Save your search strategies.
  • Link to full-text articles and other resources.
  • Use special queries and other PubMed/NCBI tools.

A short film about DNA microarrays, and how they are used to show dynamic gene expression levels.

Learn to use Entrez the main search engine for the NCBI. An understanding of the features of Entrez will improve all of your searches at the NCBI site--whether you seek genes, genomes, proteins, literature, or any of the other extensive data collections at NCBI. This is because Entrez provides a variety of tools for searching and retrieving that are common to many of the NCBI databases. Here we will review and highlight the general features of many of the most important Entrez tools.

You'll learn:

  • how to globally search all Entrez databases at once
  • to perform basic, advanced, and Boolean searches effectively
  • to interpret the results lists and understand the displays
  • about some of the more advanced Entrez tools, such as Batch Entrez and Batch Citation Matcher
  • strategies to customize and save searches to re-run later, and output mechanisms for results

This brief video provides an introductory explanation of shotgun sequencing.

This tutorial suite provides videos that explain how to get DNA sequences, use annotation tracks, locate intron-exon boundaries and search with BLAT using the UCSC Genome Browser.

The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.